| 產品名稱 |
A4.74 [4A.74] |
| 商品貨號 |
B163878 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The antibody reacts with the human fast MyHC and with rodent fast IIa MyHC. It cross-reacts weakly with IIx MyHC and does not react with fast IIb MyHC isoform of rodent skeletal muscle myosin. |
| Derivation |
Animals were immunized with partially purified human adult skeletal muscle myosin heavy chain. Spleen cells were fused with P3X63Ag8.653 mouse myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against human fast and rodent fast IIa myosin heavy chain (MyHC) |
| Cellular Products |
immunoglobulin; monoclonal antibody; against human fast and rodent fast IIa myosin heavy chain (MyHC) |
| Comments |
Animals were immunized with partially purified human adult skeletal muscle myosin heavy chain. Spleen cells were fused with P3X63Ag8.653 mouse myeloma cells. The antibody reacts with the human fast MyHC and with rodent fast IIa MyHC. It cross-reacts weakly with IIx MyHC and does not react with fast IIb MyHC isoform of rodent skeletal muscle myosin. |
| Complete Growth Medium |
Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 0.1 mM nonessential amino acids and 1 mM sodium pyruvate, 90%; iron supplemented bovine calf serum, 10%
|
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Cryopreservation |
Culture medium, 95%; DMSO, 5% |
| Isotype |
IgG1 |
| Name of Depositor |
HM Blau |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Hughes SM, Blau HM. Muscle fiber pattern is independent of cell lineage in postnatal rodent development. Cell 68: 659-671, 1992. PubMed: 1531450
Hughes SM, et al. Three slow myosin heavy chains sequentially expressed in developing mammalian skeletal muscle. Dev. Biol. 158: 183-199, 1993. PubMed: 7687223
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