Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Applications
The antibody reacts with all classes of intermediate filaments including glial filaments in astrocytes.
Tested and found negative for ectromelia virus (mousepox).
Derivation
Spleen cells were fused with NS-1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against intermediate filaments
Cellular Products
immunoglobulin; monoclonal antibody; against intermediate filaments
Comments
Animals were immunized with human glial fibrillary acidic protein.
Spleen cells were fused with NS-1 myeloma cells.
The antibody reacts with all classes of intermediate filaments including glial filaments in astrocytes.
Neurofilaments in axons.
Tonofilaments in epithelial PtK2 cells and intermediate filaments in fibroblasts.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 x 10 exp5 cells/ml and maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml.
Isotype
IgG1
Name of Depositor
R Pruss
Deposited As
mouse (B cell); mouse (myeloma)
References
Pruss RM, et al. All classes of intermediate filaments share a common antigenic determinant defined by a monoclonal antibody. Cell 27: 419-428, 1981. PubMed: 6086105
