| Applications |
The antibody binds to and inhibits the catalytic activity of rat Na,K dependent ATPase. The antibody has been used to affinity purify the enzyme, and in immunohistocytochemical localization of the enzyme in rat tissues. |
| Comments |
Animals were immunized with purified rat Na,K dependent ATPase. Spleen cells were fused with P3/NSI/1-Ag4-1 myeloma cells. The antibody binds to and inhibits the catalytic activity of rat Na,K dependent ATPase. The antibody appears to bind to the alpha subunit of the enzyme. The antibody has been used to affinity purify the enzyme, and in immunohistocytochemical localization of the enzyme in rat tissues. |
| References |
Schenk DB, et al. Use of a monoclonal antibody to quantify (Na+, K+) ATPase activity and sites in normal and regenerating rat liver. J. Biol. Chem. 259: 14941-14951, 1984. PubMed: 6094583
Sutherland E, et al. Biochemical localization of hepatic surface-membrane Na+, K+-ATPase activity depends on membrane lipid fluidity. Proc. Natl. Acad. Sci. USA 85: 8673-8677, 1988. PubMed: 2847169
Schenk DB, Leffert HL. Monoclonal antibodies to rat Na+, K+-ATPase block enzymatic activity. Proc. Natl. Acad. Sci. USA 80: 5281-5285, 1983. PubMed: 6310568
Leffert HL, et al. Hepatic (Na+, K+)-ATPase: a current view of its structure, function and localization in rat liver as revealed by studies with monoclonal antibodies. Hepatology 5: 501-507, 1985. PubMed: 2987099
Hubert JJ, et al. Rat hepatic (Na+, K+)-ATPase: alpha-Subunit isolation by immunoaffinity chromatography and structural analysis by peptide mapping. Biochemistry 25: 4156-4163, 1986. PubMed: 3017414
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