| 產品名稱 |
80 V 5B4 |
| 商品貨號 |
B163833 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
Tested and found negative for ectromelia virus (mousepox). |
| Derivation |
Animals were immunized with DEAE purified 79 X1 C2 hybridoma product conjugated to keyhole limpet hemocyanin. Spleen cells were fused with NS-1 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against an idiotypic determinant (idiotope, idiotype) on the P3X63Ag8 myeloma protein |
| Cellular Products |
immunoglobulin; monoclonal antibody; against an idiotypic determinant (idiotope, idiotype) on the P3X63Ag8 myeloma protein |
| Comments |
Animals were immunized with DEAE purified 79 X1 C2 hybridoma product conjugated to keyhole limpet hemocyanin. Spleen cells were fused with NS-1 myeloma cells. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 x 10 exp5 cells/ml and maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml. |
| Isotype |
IgG2b; kappa light chain |
| Name of Depositor |
J Gheuens |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Gheuens J, McFarlin DE. Use of monoclonal anti-idiotypic antibody to P3/X63/Ag8-myeloma protein for analysis and purification of B-lymphocyte hybridoma products. Eur. J. Immunol. 12: 701-703, 1982. PubMed: 6982818
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