| 產品名稱 |
6A7M |
| 商品貨號 |
B163804 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Strain |
Strain:? Biozzi High Responder (B cell); BALB/c (myeloma) |
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
| Derivation |
Animals were immunized with type O, Rh- human erythrocytes and purified glycophorin A. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against glycophorin A type M |
| Cellular Products |
immunoglobulin; monoclonal antibody; against glycophorin A type M |
| Tumorigenic |
Yes |
| Effects |
Yes, forms ascites in immunosuppressed BALB/c mice |
| Comments |
Animals were immunized with type O, Rh- human erythrocytes and purified glycophorin A. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody does not react with type N glycophorin A. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
|
| Subculturing |
Medium Renewal: 2 to 3 times per week Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Cryopreservation |
culture medium 95%; DMSO, 5% |
| Culture Conditions |
Temperature: 37.0°C |
| Isotype |
IgG1; kappa light chain |
| Name of Depositor |
Lawrence Livermore National Lab |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| U.S. Patent Number |
<a href="~/ref.aspx?_id=3CAE9218ABB44DB5858236D06606412C&_rf=rf">ref</a>"> (Bigbee WL, et al. Cell lines for the production of monoclonal antibodies to human glycophorin A. US Patent 4,767,710 dated Aug 30 1988) |
| References |
Bigbee WL, et al. Monoclonal antibodies specific for the M-and N-forms of human glycophorin A. Mol. Immunol. 20: 1353-1362, 1983. PubMed: 6197636
Bigbee WL, et al. Binding specificities of eight monoclonal antibodies to human glycophorin A. Studies with McM, and MkEn(UK) variant human erythrocytes and M- and MNv-type chimpanzee erythrocytes. J. Immunol. 133: 3149-3155, 1984. PubMed: 6491283
Bigbee WL, et al. Cell lines for the production of monoclonal antibodies to human glycophorin A. US Patent 4,767,710 dated Aug 30 1988
|
