| 產(chǎn)品名稱(chēng) |
3C11 |
| 商品貨號(hào) |
B163731 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Strain |
Strain:? BALB/cJ (B cell); BALB/c (myeloma) |
| Applications |
The antibody reacts with cell wall peptidoglycans from a variety of gram- and gram+ organisms. |
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
| Derivation |
Spleen cells were fused with Sp2/0-Ag14 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against bacterial cell wall peptidoglycan |
| Cellular Products |
immunoglobulin; monoclonal antibody; against bacterial cell wall peptidoglycan |
| Tumorigenic |
Yes |
| Effects |
Yes, forms ascites in pristane primed BALB/cJ mice |
| Comments |
Animals were immunized with Streptococcus mutans (BHT strain) bacterial cell wall peptidoglycan. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody reacts with cell wall peptidoglycans from a variety of gram- and gram+ organisms. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Cryopreservation |
culture medium 95%; DMSO, 5% |
| Culture Conditions |
Temperature: 37.0°C |
| Isotype |
IgG1; kappa light chain |
| Name of Depositor |
Temple University |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| U.S. Patent Number |
|
| References |
Jackson DE, et al. Monoclonal antibodies to peptidoglycan and methods of preparing same. US Patent 4,596,769 dated Jun 24 1986
|
