| Applications |
The antibody reacts with the p15 protein from several MuLV including Friend, Moloney, Rauscher, amphotropic and Spleen Focus Forming (SFFV) viruses. The antibody reacts with infected cells after acetone fixation and immunoprecipitates p15. |
| Comments |
Spleen cells from a mouse recovering from erythroleukemia induced by a B-tropic Friend murine leukemia virus were fused with NS-1 myeloma cells. The antibody reacts with the p15 protein from several MuLV including Friend, Moloney, Rauscher, amphotropic and Spleen Focus Forming (SFFV) viruses. The antibody reacts with infected cells after acetone fixation and immunoprecipitates p15. |
| References |
Chesebro B, et al. Characterization of mouse monoclonal antibodies specific for Friend murine leukemia virus-induced erythroleukemia cells: Friend-specific and FMR-specific antigens. Virology 112: 131-144, 1981. PubMed: 6787798
Chesebro B, et al. Characterization of monoclonal antibodies reactive with murine leukemia viruses: Use in analysis of strains of Friend MCF and Friend ecotropic murine leukemia virus. Virology 127: 134-148, 1983. PubMed: 6305011
Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.
Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.
Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.
Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online.
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