| 產品名稱 |
2D4 |
| 商品貨號 |
B163678 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The antibody reacts with a distinct steric portion of the carbohydrate moiety of asialo GM2, specifically the C6 hydroxyl bearing group of the terminal N-acetylgalactosamine residue. Tested and found negative for ectromelia virus (mousepox). |
| Derivation |
Spleen cells were fused with NS-1 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against asialo GM2 |
| Cellular Products |
immunoglobulin; monoclonal antibody; against asialo GM2 |
| Comments |
Animals were immunized with asialo GM2 non-covalently adsorbed to naked Salmonella minnesota. Spleen cells were fused with NS-1 myeloma cells. The antibody reacts with a distinct steric portion of the carbohydrate moiety of asialo GM2, specifically the C6 hydroxyl bearing group of the terminal N-acetylgalactosamine residue. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium |
RPMI 1640 medium, 85%; fetal bovine serum, 15%
|
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 x 10 exp5 cells/ml and maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml. |
| Isotype |
IgM |
| Name of Depositor |
WW Young |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Young WW Jr., et al. Production of monoclonal antibodies specific for two distinct steric portions of the glycolipid ganglio-N-triosylceramide (Asialo GM2). J. Exp. Med. 150: 1008-1019, 1979. PubMed: 512581
Young WW J, Hakomori SI. Therapy of mouse lymphoma with monoclonal antibodies to glycolipid: selection of low antigenic variants in vivo. Science 211: 487-489, 1981. PubMed: 7455688
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