| 產品名稱 |
1H3 |
| 商品貨號 |
B163627 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The antibody reacts with a cell surface molecule (CD62L) that mediates the initial adhesion of lymphocytes and neutrophils to endothelial cells. The antibody can be used in both immunofluorescence and immunoprecipitation assys. The cells can be adapted to serum free growth in HB101 medium. |
| Derivation |
Spleen cells were fused with Sp2/0-Ag14 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against human CD62L |
| Cellular Products |
immunoglobulin; monoclonal antibody; against human CD62L |
| Comments |
Animals were immunized with human peripheral blood leukocytes. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody reacts with a cell surface molecule (CD62L) that mediates the initial adhesion of lymphocytes and neutrophils to endothelial cells. The antibody can be used in both immunofluorescence and immunoprecipitation assys. The cells can be adapted to serum free growth in HB101 medium. |
| Complete Growth Medium |
CULTURE MEDIUM: RPMI 1640 medium, 90%; fetal bovine serum, 10%. |
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Cryopreservation |
Culture medium, 95%; DMSO, 5% |
| Isotype |
IgG2a |
| Name of Depositor |
Mo Dailey, JW Shepherd |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
. . FASEB J. 6: 1142, 1992.
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