| 產品名稱 |
171-4 |
| 商品貨號 |
B163601 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
Animals were immunized a synthetic peptide corresponding to amino acids 257 - 271 of the HLA heavy chain. The antibody reacts with a conserved epitope in the apha 3 domain of the HLA heavy chain. In Western blots, the antibody reacts with all HLA-A and HLA-B heavy chains. It is useful for quantitation of HLA-A and HLA-B antigen expression by immunoblotting. |
| Derivation |
Spleen cells were fused with Sp2/0-Ag14 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against HLA heavy chain |
| Cellular Products |
immunoglobulin; monoclonal antibody; against HLA heavy chain |
| Comments |
Animals were immunized a synthetic peptide corresponding to amino acids 257 - 271 of the HLA heavy chain. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody reacts with a conserved epitope in the apha 3 domain of the HLA heavy chain. In Western blots, the antibody reacts with all HLA-A and HLA-B heavy chains. It does not bind well to native HLA antigens. It is useful for quantitation of HLA-A and HLA-B antigen expression by immunoblotting. |
| Complete Growth Medium |
Culture Medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 90%;
fetal bovine serum, 10%. |
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Cryopreservation |
Culture medium, 95%; DMSO, 5% |
| Isotype |
IgG2a; kappa light chain |
| Name of Depositor |
K Kao |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Kao KJ, Riley WJ. Genetic predetermination of quantitative expression of HLA antigens in platelets and mononuclear leukocytes. Hum. Immunol. 38: 243-250, 1993. PubMed: 8138419
Shieh DC, Kao KJ. A combined approach for quantitation of each specific HLA-A or -B antigen expressed on cells. J. Immunol. Methods 184: 169-176, 1995. PubMed: 7658021
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