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Reconstitute plant virus by grinding the freeze dried tissue; use a mortar and pestle with 3-4 mL of a buffer such as 0.05 M sodium phosphate and 10mM sodium sulfite at pH 7.2. Rub plants with this suspension using diatomaceous earth or silicon carbide. Disease symptoms on the host plants are ususally visible within two weeks

Dracopoli NC, et al. The complete nucleotide sequence of the genomic RNA of the tobamovirus tobacco mild green mosaic virus. Virology 177: 553-558, 1990. PubMed: 2371769

Valverde RA, et al. Interactons between satellite tobacco mosaic virus, helper tobamoviruses, and their hosts. Phytopathology 81: 99-104, 1991.

Mundry KW, et al. Complete uncoating of the 5' leader sequence of tobacco mosaic virus RNA occurs rapidly and is required to initiate cotranslational virus disassembly in vitro. J. Gen. Virol. 72: 769-777, 1991. PubMed: 1849966