| 產(chǎn)品名稱(chēng) |
Canine parvovirus |
| 商品貨號(hào) |
B163251 |
| Classification |
Parvoviridae, Parvovirus, Feline panleukopenia virus |
| Agent |
Canine parvovirus |
| Strain |
C-780916 |
| Applications |
Antigenically indistinguishable from feline panleukopenia virus and mink enteritis virus, but it has a broader host range and different HA spectrum, especially as regards pH conditions. Hemagglutinates swine, Rh+ monkey & cat RBC at pH 6.0-7.5. On passage, add virus 2 hrs after setting host cells. Antigenically indistinguishable from feline panleukopenia virus and mink enteritis virus, but it has a broader host range and different HA spectrum, especially as regards pH conditions. |
| Biosafety Level |
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation |
Feces of dog with hemorrhagic enteritis |
| Product Format |
frozen 1 mL per vial |
| Storage Conditions |
-70°C or colder |
| Comments |
Non-pathogenic for neonatal pups. Antigenically indistinguishable from Feline panleukopenia virus and Mink enteritis virus, but has broader host range and different HA spectrum, especially in regards to pH conditions. Hemagglutinates porcine, Rh+ monkey, and feline red blood cells at pH 6.0-7.5. May fail to immunize dogs if passaged 8-10 times in feline cells. |
| Effect on Host |
CPE, refractile cells, cell rounding and sloughing |
| Recommended Host |
A-72 (ATCC® CRL-1542™) |
| Growth Conditions |
Recommendations for Infection: Plate cells 2-4 hours prior to infection and infect when cultures are 30-40% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 35°C in a humidified atmosphere, rocking every 15 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: Incubate infected culture for 3-9 days at 35°C in a humidified atmosphere, until CPE are well advance throughout.
Virus Growth Medium: Leibovitz's L-15 Medium (ATCC 30-2008) + 2% FBS (ATCC 30-2020) |
| Name of Depositor |
LE Carmichael, New York State College of Veterinary Medicine, Cornell University |
| Source |
Feces of dog with hemorrhagic enteritis, 1978 |
| References |
Appel MJ, et al. Isolation and immunisation studies of a canine parco-like virus from dogs with haemorrhagic enteritis. Vet. Rec. 105: 156-159, 1979. PubMed: 516347
Carmichael LE, et al. A modified live canine parvovirus strain with novel plaque characteristics. I. Viral attenuation and dog response. Cornell Vet. 71: 408-427, 1981. PubMed: 7318443
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