| 產(chǎn)品名稱 |
Murine leukemia virus |
| 商品貨號(hào) |
B163216 |
| Classification |
Retroviridae, Gammaretrovirus, Mammalian virus group |
| Agent |
Murine leukemia virus |
| Strain |
AKR 13 MCF |
| Applications |
Typical retrovirus: buoyant density 1.16 g/cc in sucrose, lipid-containing envelope, infectivity rapidly destroyed by freezing and thawing; neutralized by antiserum to both xenotropic and AKR ecotropic viruses; possesses gs-1 antigen of MuLV. Typical retrovirus: buoyant density 1.16 g/cc in sucrose, lipid-containing envelope, infectivity rapidly destroyed by freezing and thawing; neutralized by antiserum to both xenotropic and AKR ecotropic viruses; possesses gs-1 antigen of MuLV. |
| Biosafety Level |
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation |
AKR/J mouse (thymus) co-cultivated with Mv-1 Lu cells (ATCC CCL 64) |
| Product Format |
frozen |
| Storage Conditions |
-70°C or colder |
| Comments |
Typical retrovirus: buoyant density 1.16 g/cc in sucrose, lipid-containing envelope, infectivity rapidly destroyed by freezing and thawing; neutralized by antiserum to both xenotropic and AKR ecotropic viruses; possesses gs-1 antigen of MuLV. This preparation has tested positive for A. laidlawii. Typical retrovirus: buoyant density 1.16 g/cc in sucrose, lipid-containing envelope, infectivity rapidly destroyed by freezing and thawing; neutralized by antiserum to both xenotropic and AKR ecotropic viruses; possesses gs-1 antigen of MuLV. |
| Effect on Host |
tumorigenicity: Accelerated leukemia in AKR mice no CPE in Mv-1 Lu cells.? Assay in MiCl cells (ATCC CCL-64.1), produces CPE foci |
| Recommended Host |
Mv 1 Lu (mink lung) cells (ATCC CCL-64) Host of Choice: Mv-1 Lu cells (ATCC CCL-64) Host Range: AKR mouse, Mv-1 Lu cells, MiCl cells AKR mouse; Mv 1 Lu cells (ATCC CCL-64) |
| Growth Conditions |
Temperature: 37.0°C |
| Name of Depositor |
JW Hartley |
| Source |
AKR/J mouse (thymus) co-cultivated with Mv-1 Lu cells (ATCC CCL 64) |
| References |
Elder JH, et al. Biochemical evidence that MCF murine leukemia viruses are envelope (env) gene recombinants. Proc. Natl. Acad. Sci. USA 74: 4676-4680, 1977. PubMed: 200928
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