| 產品名稱 | IMR-90 |
|---|---|
| 商品貨號 | B162647 |
| Organism | Homo sapiens, human |
| Tissue | lung |
| Cell Type | fibroblast |
| Product Format | frozen |
| Morphology | fibroblast |
| Culture Properties | adherent |
| Biosafety Level | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | normal |
| Age | 16 weeks gestation |
| Gender | female |
| Ethnicity | Caucasian |
| Applications | This cell line is a suitable transfection host. |
| Storage Conditions | liquid nitrogen vapor phase |
| Karyotype | normal human female; diploid; stable. Note: Cytogenetic information is based on initial seed stock at ATCC. Cytogenetic instability has been reported in the literature for some cell lines. |
| Images | 
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| Derivation | The human diploid fibroblast strain IMR-90 was derived by W.W. Nichols and associates from the lungs of a 16-week female fetus. |
| Clinical Data | 16 weeks gestation
Caucasian
female
|
| Virus Susceptibility | Human poliovirus 1
Human poliovirus 2 Varicella-Zoster Herpes simplex virus 1 Herpes simplex virus 2 Human poliovirus 3 Vaccinia virus Human herpesvirus 5 , Human cytomegalovirus Vesicular stomatitis virus |
| Comments | The division potential, viral susceptibilities and other properties have been thoroughly studied such that the line may be considered as an alternate for WI-38 and other standard human lung cell strains. The cells have been reported to be capable of attaining 58 population doublings before the onset of senescence. |
| Complete Growth Medium | The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Corning® T-75 flasks (catalog #430641) are recommended for subculturing this product.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:8 is recommended
Medium Renewal: Every 3 to 4 days |
| Cryopreservation | Freeze medium: Complete growth medium 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions | Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C |
| STR Profile | Amelogenin: X CSF1PO: 11,13 D13S317: 11,13 D16S539: 10,13 D5S818: 12,13 D7S820: 9,12 THO1: 8,9.3 TPOX: 8,9 vWA: 16,19 |
| Isoenzymes | G6PD, B |
| Name of Depositor | WW Nichols |
| Deposited As | Homo sapiens |
| References | Nichols WW, et al. Characterization of a new human diploid cell strain, IMR-90. Science 196: 60-63, 1977. PubMed: 841339 Dolganov GM, et al. Human Rad50 is physically associated with human Mre11: identification of a conserved multiprotein complex implicated in recombinational DNA repair. Mol. Cell. Biol. 16: 4832-4841, 1996. PubMed: 8756642 Ostlund RE Jr., et al. A sterospecific myo-inositol/D-chiro-inositol transporter in HepG2 liver cells. J. Biol. Chem. 271: 10073-10078, 1996. PubMed: 8626564 |
| 梅經理 | 17280875617 | 1438578920 |
| 胡經理 | 13345964880 | 2438244627 |
| 周經理 | 17757487661 | 1296385441 |
| 于經理 | 18067160830 | 2088210172 |
| 沈經理 | 19548299266 | 2662369050 |
| 李經理 | 13626845108 | 972239479 |
