| 產品名稱 |
HFBDN02 |
| 商品貨號 |
B162614 |
| Designations |
HFBDN02 |
| GenBank Number |
T06019
|
| Species |
Homo sapiens, human |
| Vector |
Construct size (kb): 5.0 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ', <-
primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, <-
promoter: lac, <-
primer site: M13 reverse, <-
replicon: pMB1
marker(s): ampR, <-
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: GOT2
Genomic copy number: unique
Gene name: glutamic-oxaloacetic transaminase 2, mitochondrial
(aspartate aminotransferase 2)
Contains complete coding sequence?: N
Chromosome: 16; Localization: 16 q13
Tissue: fetal brain, female, 17-18 wk (Stratagene 936206)
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 2.0
Cross references: DNA Seq. Acc.: M22632; EST: EST03908
Nucleotides 85-303 of the insert correspond to
nucleotides 2137-1919 of M22632 with 97% identity.
DNA Seq. Acc.: T06019; EST: EST03908; Primer: M13-21 Insert lengths(kb): 2.0 Tissue: fetal brain, female, 17-18 wk (Stratagene 936206) Gene product: glutamic-oxaloacetic transaminase 2, mitochondrial (aspartate aminotransferase 2)( Glutamate oxaloacetate transaminase 2, mitochondrial (aspartate aminotransferase 2), mitochondrial aspartate aminotransferase) [GOT2] |
| Insert Size (kb) |
2.0 |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 2.0. Based on the alignment with GenBank M22632 (2339 nt, CDS 9-1301) and the insert size, this clone probably contains some of the coding sequence and most of the 3' untranslated region. EST03908 contains a Line 1 element: 1-85. |
| References |
Adams MD, et al. 3400 expressed sequence tags identify diversity of transcripts from human brain. Nat. Genet. 4: 256-267, 1993. PubMed: 8358434
|
