| 產品名稱 |
IgG-2F1 |
| 商品貨號 |
B162273 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
Animals were immunized against GST-Rab escort protein-1 (REP-1). The antibody is specific for REP-1 from human lymphoblastoid cell lines and peripheral blood leukocytes. It is useful for detecting the level of REP-1.
This antibody is used as a diagnostic tool for choroideremia (CHM), an X-linked form of retinal degeneration, by immunoblot analysis. |
| Derivation |
Spleen cells were fused with Sp2/0-Ag14 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against human REP-1 |
| Cellular Products |
immunoglobulin; monoclonal antibody; against human REP-1 |
| Comments |
Animals were immunized against GST-Rab escort protein-1 (REP-1). Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody is specific for REP-1 from human lymphoblastoid cell lines and peripheral blood leukocytes. It is useful for detecting the level of REP-1.
This antibody is used as a diagnostic tool for choroideremia (CHM), an X-linked form of retinal degeneration, by immunoblot analysis. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density) Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10 exp5 viable cells/ml. Maintain cultures between 5 X 10 exp4 and 5 X 10 exp5 cells/ml. |
| Cryopreservation |
Culture medium, 95%; DMSO, 5% |
| Culture Conditions |
Temperature: 37.0°C |
| Isotype |
IgG1 |
| Name of Depositor |
JL Goldstein, YK Ho |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
MacDonald IM, et al. A practical diagnostic test for choroideremia. Ophthalmology 105: 1637-1640, 1998. PubMed: 9754170
Andres DA, et al. Mutational analysis of alpha-subunit of protein farnesyltransferase. Evidence for a catalytic role. J. Biol. Chem. 268: 1383-1390, 1993. PubMed: 8419339
Seabra MC, et al. Purification of component A of Rab geranylgeranyl transferase: possible identity with the choroideremia gene product. Cell 70: 1049-1057, 1992. PubMed: 1525821
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