| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--22.3, 11.0, 6.2, 2.4, 1.95, 0.95; EcoRI/HindIII--24.0, 9.5, 4.1, 1.8, 1.8, 1.0, 0.9. Verified to contain the correct insert sequence using the following oligonucleotides (5'-3'): GTGTGGAGAGGCATTCATTCGAAGC and TTCTATTGGAACAGAATGCTCTCCC. The predicted product size is (bp): 113. The observed product size is (bp): 113. |
