| 產(chǎn)品名稱 |
LL/2-Luc2 |
| 商品貨號 |
B161692 |
| Organism |
Mus musculus, mouse |
| Product Format |
frozen 1.0 mL |
| Morphology |
rounded - loosely attached or floating |
| Culture Properties |
mixed, adherent and suspension |
| Biosafety Level |
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
Lewis lung carcinoma |
| Strain |
C57BL |
| Applications |
Excellent signal/background ratio and stable luciferase expression make this cell line ideal for in vivo bioluminescence imaging of xenograft animal model to study human cancer and monitor activity of anti-cancer drug. It also can be used in cell-based assays for cancer research. |
| Storage Conditions |
liquid nitrogen vapor phase |
| Tumorigenic |
Yes, tested in C57BL mice |
| Comments |
This luciferase expressing cell line was derived from parental line ATCC CRL-1642 by transduction with lentiviral vector encoding firefly luciferase gene (luc2) under control of EF-1 alpha promoter. This cell line was established through single cell cloning, and the cells constitutively express high levels of enzymatically active luciferase protein, which can be detected via in vitro and in vivo bioluminescence assays. The cells should be maintained in blasticidin (2 μg/mL) containing medium in routine cell culture. It is recommended to remove blasticidin prior to and during the experiment procedure when the cells are injected into animals in vivo, or co-cultured with other cell types in vitro. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium (DMEM; ATCC 30-2002). To make the complete growth medium, add the following components to the base medium:
- Fetal bovine serum (FBS; ATCC 30-2020) to a final concentration of 10%
- Blasticidin to a final concentration of 2 µg/mL
|
| Subculturing |
Subcultures are prepared by diluting the suspension 1:4 to 1:6. Cells on the floor of the flask may be dislodged by aspirating several times with culture medium or by rinsing with 0.25% trypsin - 0.53 mM EDTA solution. Corning® T-75 flasks (catalog #430641) are recommended for subculturing this product. Cultures can be established between 4 x 104 and 8 x 104 viable cells/cm2. Incubate cultures at 37°C.
Interval: Maintain cultures at a cell concentration between 4 X 104 and 3.5 X 105 cell/cm2.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week |
| Cryopreservation |
Complete growth medium supplemented with 5% (v/v) DMSO (ATCC 4-X) |
| Culture Conditions |
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C |
| Cells per Vial |
≥ 1.0 x 106 cells |
| Volume |
1.0 mL |
| Sterility Tests |
Bacteria and yeast: No growth
Mycoplasma: No growth |
| Functional Tests |
Luciferase activity: signal to noise ≥ 1,000 RLUs
In vitro luminesence: 10,000 photons/cell/sec, subject to imaging and culturing conditions |
| Population Doubling Time |
approximately 13 hours |
| Name of Depositor |
ATCC |
| Year of Origin |
2018 |
| References |
Zinn KR, et al. Noninvasive bioluminescence imaging in small animals. ILARJ 49: 103-115, 2008. PubMed: 18172337
Dothager RS, et al. Advances in bioluminescence imaging of live animal models. Curr Opin Biotechnol 20: 45-53, 2009. PubMed: 19233638
Bertram JS, Janik P. Establishment of a cloned line of Lewis lung carcinoma cells adapted to cell culture. Cancer Lett. 11: 63-73, 1980. PubMed: 7226139
Sharma S, et al. T cell-derived IL-10 promotes lung cancer growth by suppressing both T cell and APC function. J. Immunol. 163: 5020-5028, 1999. PubMed: 10528207
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